TEST PROCEDURE FOR CRUDE FIBRE ESTIMATION

HOW TO FIND CRUDE FIBRE IN FOOD PRODUCT

REAGENTS

1. Dilute sulphuric acid - 1.25 % m/v, accurately prepared

2. Sodium hydroxide solution - 1.25 % m/v. accurately prepared

APPARATUS

1. Electric air Oven

2. Thimble

3. Soxhlet apparatus

4. Reflux condenser

5. Gooch crucible

6. Muffle furnace

PROCEDURE

1. Dry to constant mass about 5 g of the material in an electric air oven at 105 to 110 deg C. Weigh accurately about 2.5 g of the dried material into a thimble and extract for about one hour with petroleum ether, using a soxhlet apparatus.

2. Transfer the fat-free material to a one-litre flask.

3. Take 200 ml of dilute sulphuric acid in a beaker and bring to boil. Transfer fat-free material and immediately connect the flask with a reflux water condenser and heat, so that the contents of the flask begin to boil within one minute.

4. Rotate the flask frequently, taking care to keep the material from remaining on the sides of the flask out of contact with the acid. Continue boiling for exactly 30 minutes.

5. Remove the flask and filter through fine linen and silk cloth (75 micron) held in a funnel, and wash with boiling water until the washings are no longer acid to litmus.

6. Bring to boil some quantity of sodium hydroxide solution under a reflux condenser.

7. Wash the residue on the linen into the flask with 200 ml of the boiling sodium hydroxide solution. Immediately connect the flask with the reflux condenser and boil for exactly 30 minutes Remove the flask and immediately filter through the filtering cloth.

8. Thoroughly wash the residue with boiling water and transfer to a Gooch crucible prepared with a thin but compact layer of ignited asbestos

9. Wash the residue thoroughly first with hot water and then with about 15 ml of ethyl alcohol, 95 % by volume.

10. Dry the Gooch crucible and contents at 105 - 110 deg C in an air - oven to constant mass. Cool and weigh

11. Incinerate the contents of the Gooch crucible in an electric muffle furnace at 600 ± 20 deg C until all the carbonaceous matter is burnt. Cool the Gooch crucible containing the ash in a dessicator and weigh.

Calculation

Crude fibre (on dry basis) % by mass =

100 (M1 - M2)

Where,

M1 = Mass in gm of Gooch crucible and contents before ashing

M2 = Mass in gm of Gooch crucible containing asbestos and ash, and

M = Mass in gm of the dried material taken for the test

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