DETERMINATION OF FAT CONTENT

SCOPE: All raw materials and finished products

SAMPLE PREPARATION

1. Mix thoroughly the sample & place it in a mixer grinder.

2. Operate the grinder for 10 seconds.

3. With the help of scoop mix well the content and operate further for five seconds.

4. Mix thoroughly and make it uniform mass on a butter paper.

APPARATUS

1. Soxhlet extraction apparatus.

2. Air - oven. (0 - 105 °C).

3. Glass - flat bottom flask - 250 ml

4. Whatman Cellulose Extraction thimbles 25 mm x 80 mm.

5. Desiccator

6. Metler Electronic Balance (L.C - 0.1 mg.)

REAGENTS

Petroleum ether 60 /80 °C.

PROCEDURE

1. Carefully weigh about 20 g of well-mixed powdered sample in an extraction thimble.

2. Plug the thimble with absorbent cotton, ensuring that no material comes out.

3. Place the thimble in the extractor and fit it to the previously weighed flat bottom flask.

4. Add about 200 ml of solvent to the flat bottom flask (which contains glass beads). Affix the condenser and allow through it a current of tap water to flow and heat the flask.

5. Continue the extraction for 6 hours.

6. Remove the extractor from its bath, take out the extraction thimble, and drain the solvent portion in the flask.

7. Keep the Extraction thimble on the neck of the flask for approximately 5 - 10 minutes so that complete draining of the solvent takes place.

8. Now remove the greater part of solvent in flask by distillation from a hot - water bath.

9. Heat the flask in an oven at 105 °C ± 2 ° C for one hour to remove solvent.

10. Remove the flask from oven and keep it in a desiccator to cool and weigh the flask.

CALCULATION

Fat % = W2 - W1 x 100

W2 = Weight of the flask after cooling

W1 = Weight of the flask before extraction

W = Weight of the sample

Comments

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